DNA & RNA Purification Kits
abm offers a variety of specialty DNA and RNA kits allowing for efficient extraction from various sources such as animal, plant, blood, bacteria and fungi. Additionally you can find affordable and reliable kits for routine techniques such as Gel & PCR clean up purification, as well as specialized techniques such as circRNA isolation.
DNA Extraction and Purification Kits
Column-Pure Plasmid Miniprep Kit
Column-Pure Gel and PCR Clean-Up Kit
DNA Purification Magnetic Beads
RNA Extraction and Purification Kits
Column-Pure RNA Miniprep Kit
Column-Pure Fungi RNA Isolation Kit
RNA Purification Magnetic Beads
circRNA Isolation Kit
Genomic DNA Extraction Kits
Column-Pure Blood Genomic DNA Kit
Column-Pure Bacterial Genomic DNA Isolation Kit
Column-Pure Animal Genomic DNA Isolation Kit
Column-Pure Plant DNA Extraction Kit
RNA Tracking & Visualization (RNA Mango)
The most advanced RNA tracking, visualization and pull down technology
Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.
Key Features
RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango aptamer (Fluorescent enhancement FE=1,100).
The Thizole Orange (TO) dye has a number of other desirable properties including:
- Small size
- Lack of toxicity
- Plasma and nuclear membrane permeability
- Short intracellular half-life
- The accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure
- The TO1 and TO3 dyes can be used in a 2-color reporter assay system using both the RNA Mango and RNA Peach aptamer systems (Kong et. al. 2021)
TO1-biotin is the standard variety of TO dye for RNA Mango experiments
RNA Mango in Action
Transcription reaction were carried out in 300 µL volumes using T7 RNA polymerase (400 U, 50U/µL, applied biological materials), 0.5 µM TO1-3PEG-Biotin (applied biological materials), in 8 mM GTP, 5 mM CTP and ATP, 2 mM UTP, 40 mM TRIS buffer pH 7.9, 2.5 mM spermidine, 26 mM MgCl2, 20 mM KCl, Pyrophosphatase (0.5 U, 0.1 U/µL, ThermoFisher Scientific), and 0.01% Triton X-100. To each sample, either water (Negative), 0.33 µM DNA template (Mango Transcription), or 500 nM final Mango III A10U RNA (Positive) was added. Samples were visualized in a blue light box, movie is played back at 30X speed.