Expression-Ready Libraries
Lentiviral Vectors & Virus
As a leader in lentiviral technology, abm offers an affordable, comprehensive collection of human, mouse and rat genes cloned into ready-to-use lentiviral vectors or packaged recombinant lentivirus for gene over-expression studies within a wide range of host cells.
Advantages of the Lentivirus System:• Large insert capacity (up to 9kb)
• High infection efficiency
• Broad host range (dividing, non-dividing, stem cells, and primary cells)
• Stable integration & expression of recombinant proteins
• Ideal for the creation of stable cell lines
Lentivirus Workflow
View our simplfied workflow with ready-to-use lentiviral vectors and viruses.
Achieve Up To 300 Fold Over-Expression
qPCR amplification of OVOL1 gene following transfection with abm's OVOL1 Lentiviral Vector.
Lentivirus Introduction
Learn more about the Lentivirus system by visiting our Learning Resources.
AAV Vectors & Virus
As a leader in Adeno-Associated Virus (AAV) technology, abmoffers an affordable, comprehensive collection of human, mouse and rat genes cloned into ready-to-use AAV vectors or packaged AAV virus (Serotypes 1-11) for gene over-expression studies within a wide range of host cells.
Advantages of AAV System:• Used as a promising candidate for gene therapy
• Does not elicit significant immune responses in vivo
• Broad tropism - tissue specificity with different AAV serotypes
• No integration into the host genome
• Ability to transduce both proliferating and quiescent cells
• Long-term expression in non-dividing cells
AAV Serotype Selection Chart
Try AAV Serotype Blast Kit to optimize your experiments.
The Adeno-Associated Virus
Learning Resources
Supporting Data
EGFP expression in lumbar neuronal cells 4 weeks after intrathecal injection into mice (Left), and seen with β-tubulin (red) and DAPI (blue) overlay (Right). Courtesy of Dr. D Lopes, King's college London.
Adenovirus
abm offers an affordable, comprehensive collection of premade adenoviruses containing full-length ORFs for human, mouse, and rat genes for gene over-expression studies. Standard Adenovirus Collection constructs contain the strong CMV promoter with the option of HA or His tags.
Advantages of the Adenovirus System:• High transduction efficiency in most mammalian cells
• Low immunogenicity for high post-infection cell viability
• Non-integrating and no risk of disrupting host genes
• Broad host range (dividing, non-dividing, stem cells, and primary cells)
• High titers can be obtained
• Large insert capacity (up to 6 kb)
• A popular choice in vivo and in vitro applications
• Biosafety: abm use replication-incompetent (-E1/-E3) human adenovirus type 5 (Ad5)
siRNA Technology
siRNAs are powerful tools for gene silencing and can be delivered to the target cell or host via a variety of methods including ready-to-use recombinant lentivirus and AAV or direct transfection of DNA plasmids or double stranded RNA (dsRNA). Our unique convergent dual promoter system avoids the need to design hair-pin loop shRNA structures, streamlining cloning and knockdown of the target gene. Check out comprehensive collection of human, mouse, and rat siRNAs pre-designed for knockdown of your gene of interest.
Products: siRNA Lentivirus, siRNA AAV, siRNA dsRNA Oligo
miRNA Technology
abm offers a variety of miRNA products to inhibit or over-express any miRNA for studies of post-transcriptional gene regulation in mammalian systems using our ready-to-use viral vectors and packaged viruses, as well as our detection and quantification tools.
ORF Vector Collection
abm offers an extensive collection of sequence verified human, mouse and rat Open Reading Frame (ORF) genes, ready for insertion into your choice of vector expression system. The ORFs are cloned into a small non-viral vector containing a mutated stop codon to allow for the incorporation of a C-terminal tag. The ORFs do not contain the 5’ or 3’ UTRs which enable for quick and reliable protein expression when inserted into a compatible expression vector.
circRNA Technology
abm is the leader in circRNA research and product development. This emerging technology has garnered us a niche spotlight with circRNA researchers around the world. Comprehensive product line offers circRNA over-expression vectors, purification kits and enzymes including our unique RNase R enzyme.
circRNAs can be over-expressed in mammalian cells by using non-repetitive regions of reverse complementary sequences - these work to bring the exon-flanking portion of introns in close proximity, thus promoting back-splicing into circRNA. abm offers a collection of over 1000 circRNA over-expressing non-viral and AAV vectors. These discovered circRNAs are associated with human disease and can be used in over-expression studies to investigate new roles of circRNA in health.
Control Vectors and Viruses
At abm, we know that every experiment requires an appropriate control. We offer a variety of vector and virus controls including CRISPR Scrambled Controls, siRNA Scrambled Controls and Gene Expression Controls (empty control vectors) for all of our viral and non-viral expression systems.
Products: circRNA Controls, Adenovirus Controls, miRNA Controls, AAV Controls, Lentivirus Controls, CRISPR Controls, Retrovirus Controls