CRISPR Products for Genome Editing
abm, a leader in genetic engineering tool development for the last 15 years, holds a CRISPR license with ERS, co-founded by 2020 Nobel Prize winner Dr. Emmanuelle Charpentier, granting access to the ERS CRISPR/Cas9 patent portfolio, enabling us to offer a full line of CRISPR Products for research.This portfolio has more than 55 patents in the US covering the use of CRISPR/Cas9 gene editing in all cells, including eukaryotic. Companies based in the US, using CRISPR/Cas9 will require a CVC license.
CRISPR sgRNA Library
abm's CRISPR Gene Knockout sgRNA vectors and viruses are highly effective at achieving knockout of your target gene. Cas9 functions to create a double-stranded break within an early exon triggering repair via Non-Homologous End-Joining (NHEJ) mechanism resulting in deleterious frameshift mutations. abm offers a comprehensive collection of All-in-One (spCas9 and sgRNA expressing) and sgRNA only expressing constructs targeting any human, mouse, or rat gene.
CRISPR Activation
abm's CRISPR Activation sgRNA vectors and viruses are highly effective at achieving activation of your target gene. CRISPR Activation (CRISPRa) allows for gene specific up-regulation by using dCas9-VPR. This fusion protein consists of the catalytically dead Cas9 (dCas9) enzyme fused to the tripartite complex VPR (VP64, p65 and Rta). abm offers a comprehensive collection of sgRNA-only lentiviral vectors expressing sgRNAs that target the upstream 5' UTR promoter region of any human, mouse or rat gene thereby resulting in transcriptional up-regulation.
Cas9 Expression Vectors and Viruses
abm's offers an extensive collection of Cas9 vectors and viruses, from spCas9 and saCas9 nucleases to null mutants and nickases, in a variety of viral delivery systems. Pair abm's Cas9 products with a compatible sgRNA expression vector/virus from our ready-to-use sgRNA KO Library or sgRNA Activation Library. Products: Cas9 Vectors and Viruses, sgRNA Vectors and Viruses
Cas Proteins & CRISPR Screening
CRISPR is the most versatile technology for genome editing and abm offers the largest selection of Cas9 proteins for CRISPR experiments. Cas9 Ribonucleoproteins (RNPs) are Cas9 proteins in complex with in vitro transcribed sgRNA. These RNPs can be used for in vitro pre-screening sgRNA candidates and can be directly transfected or electroporated into cells to achieve gene editing. Advantages of Cas9 RNP Delivery Method:• No transcription or translation required, thus editing is rapid and transient
• No plasmid or virus is used, therefore no risk of genome integration of Cas9/sgRNA machinery
• Proteins are compatible with a wide range of organisms, no need to consider promoters & codon optimizationabm is continually developing a range of CRISPR-Cas proteins, while also advancing high-fidelity options for precise genetic editing. Products: spCas9 Nuclease, spCas9 Nickase, spCas9 Null Mutant, spCas9 GFP, saCas9 Nuclease, CRISPR Kits